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Transformation of white spruce (Picea glauca) somatic embryos by microprojectile bombardment

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1007/BF00232308
AuthorSearch for: 1; Search for: 1; Search for: 1; Search for: 1
Affiliation
  1. National Research Council Canada. NRC Plant Biotechnology Institute
FormatText, Article
Subjectgene expression; blot analysis; somatic embryo; southern blot; fusion gene
Abstract
Cotyledonary somatic embryos of white spruce [Picea glauca (Moench) Voss] were subjected to microprojectile bombardment with a gene construct containing a gus::nptll fusion gene. Somatic embryos were used to re-induce the embryogenic tissue after bombardments. Histochemical assay using X-gluc as a substrate showed that all the embryos (100%) were GUS positive 48 h after bombardment. However, only thirteen out of 605 embryos (2.2%) remained GUS positive after two months in culture. Three of those thirteen (23%) embryo-derived tissues consistently showed GUS activity for eight months in culture. These putatively transfomed embryogenic tissues were subjected to Southern blot analysis and the results suggested integration of the gus::nptll gene expression cassette in the white spruce genome.
Publication date
PublisherSpringer Nature
In
  • Plant Cell Reports 13, no. 1 (November 1993).
LanguageEnglish
Peer reviewedYes
IdentifierBF00232308
NRCC-36495
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Record identifier550b6185-0282-412a-8d66-6e6f398ac467
Record created2023-11-06
Record modified2023-11-06
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