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Solution conformation of αD(1-3)- and αD(1-6)-linked oligomannosides using proton nuclear magnetic resonance

From National Research Council Canada

Alternative titleSolution conformation of alpha D(1-3)- and alpha D(1-6)-linked oligomannosides using proton nuclear magnetic resonance
DOIResolve DOI: https://doi.org/10.1021/bi00275a007
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FormatText, Article
Subject13C; 1H; alpha; asparagine-linked; calculation; calculations; carbohydrate conformation; conformation; conformations; coupling; coupling constants; coupling-constants; crystal; crystal-structure; crystal-structures; energy; energy calculations; glycopeptide; glycopeptides; glycosides; linkages; magnetic; magnetic resonance spectroscopy; magnetic-resonance; mannose; mannosides; methods; methyl; models,molecular; N-linked; nuclear; Nuclear Magnetic Resonance; Overhauser; potential; proton; relaxation; resonance; solution; solution conformation; solutions; structure; synthetic; technique; thermodynamics; unit
Abstract
The solution conformations of synthetic methyl mannobiosides and a methyl mannotrioside containing α D(1-3) and α D(1-6) linkages have been determined through the use of ¹H nuclear magnetic resonance techniques, namely, the nuclear Overhauser effect and proton relaxation time measurements. ³J(C,H) coupling constants, obtained from a compound enriched with ¹³C, were also used. The allowed conformations were found to be in agreement with those determined from potential energy calculations and crystal structures. The methyl mannotrioside is an analogue of a mannotriose unit which occurs naturally in the "core" of asparagine-linked glycopeptides and in an "arm" of high mannose N-linked glycopeptides
Publication date
PublisherAmerican Chemical Society
In
  • Biochemistry 22, no. 6: 1362–1368.
LanguageEnglish
Peer reviewedYes
NRC publication
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IdentifierNRC-IBS-BRISSON1983I
NPARC number9363346
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Record identifier5303f014-e657-4a26-bf89-f1cec41e5936
Record created2009-07-10
Record modified2020-03-13
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