| DOI | Resolve DOI: https://doi.org/10.1103/PhysRevLett.95.208102 |
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| Author | Search for: Greenleaf, William; Search for: Woodside, Michael1; Search for: Abbondanzieri, Elio; Search for: Block, Steven |
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| Affiliation | - National Research Council Canada. National Institute for Nanotechnology
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| Format | Text, Article |
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| Subject | biological techniques; biomechanics; DNA; laser beam effects; molecular biophysics; optical feedback; radiation pressure; stability |
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| Abstract | Optical traps are useful for studying the effects of forces on single molecules. Feedback-based force clamps are often used to maintain a constant load, but the response time of the feedback limits bandwidth and can introduce instability. We developed a novel force clamp that operates without feedback, taking advantage of the anharmonic region of the trapping potential where the differential stiffness vanishes. We demonstrate the utility of such a force clamp by measuring the unfolding of DNA hairpins and the effect of trap stiffness on opening distance and transition rates. |
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| Publication date | 2005-11-11 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 12338342 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 52b3e760-b457-41c5-8107-61969b6cd4f9 |
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| Record created | 2009-09-10 |
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| Record modified | 2020-04-07 |
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