National Research Council of Canada. Medical Devices
18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, October 26-30 2014, San Antonio, Texas, USA
Magnetic capture; Bacteria; Polymer microfluidic device; Array of pillars
An integrated polymer-based microfluidic device for capture and release of bacteria using antibodyspecific silica-shell-iron-oxide-core nanoparticles is presented. The magnetic capture-release chip (MCR) relies on a dense array of Nickel-coated high-aspect ratio pillars and a microfluidic channel network for sample delivery, capture, wash and recovery. The unique characteristic of the MCR lies in the ability to control the magnetic field distribution within the chamber without consequent remanence, thus allowing sample capture and release. The capture efficiency was validated using plate-culture method and the minimum detectable concentration for short 15 min bacteria-nanoparticle incubation corresponded to 10 cfu/ml for Listeria monocytogenes.
Chemical and Biological Microsystems Society
18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2014)1: 2546–2547.