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Cryopreservation of periwinkle, Catharanthus roseus cells cultured in vitro

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1007/BF00269181
AuthorSearch for: 1; Search for: 1; Search for: 1; Search for: 1
Affiliation
  1. National Research Council of Canada. Prairie Regional Laboratory
FormatText, Article
Abstract
A procedure for prolonged cryogenic storage of periwinkle cell cultures is described. Cells derived from periwinkle, Catharanthus roseus (L.) G. Don, and subcultured as suspension in 1-B5C nutrient medium have been frozen, stored in liquid nitrogen (−196°C) for 11 weeks, thawed and recultured. Maximal survival was achieved when 3–4 day-old cells precultured for 24 h in nutrient medium with 5% DMSO were frozen at slow cooling rates of 0.5 or 1°C/min prior to storage in liquid nitrogen. The only loss in viability of cells occurred subsequent to treatment with DMSO. Abbreviations: DMSO, dimethylsulfoxide; 2,4-D, 2,4-dichlorophenoxyacetic acid; TTC, triphenyltetrazolium chloride.
Publication date
PublisherSpringer Nature
In
  • Plant Cell Reports 1, no. 4 (June 1982): 135–138.
LanguageEnglish
Peer reviewedYes
IdentifierBF00269181
NRCC-20082
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Record identifier4a4dee09-c3b3-48cc-9c34-25d5a89aaf7e
Record created2023-11-16
Record modified2023-11-16
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