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Structure of the glycan chain from the surface layer glycoprotein of Bacillus alvei CCM 2051

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1139/o91-010
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FormatText, Article
Subjectsurface layer; eubacteria; glycoprotein; nuclear magnetic resonance; structure
Abstract
The cell surface of the mesophilic eubacterium Bacillus alvei CCM 2051 is covered by an oblique arranged surface layer glycoprotein. The subunits revealed by sodium dodecyl sulfate - polyacrylamide gel electrophoresis were distinct bands of molecular masses 140,000, 128,000, and 127,000. Proteolytic degradation of the purified S-layer glycoprotein yielded a single glycopeptide fraction with an apparent molecular mass of ca. 25,000. Methylation analysis in conjunction with two-dimensional nuclear magnetic resonance experiments at 500 MHz established the branched trisaccharide (Formula: see text) as the repeating unit for this glycan chain.
Publication date
PublisherNational Research Council of Canada. NRC Research Press
In
  • Biochemistry and Cell Biology 69, no. 1: 72–78.
LanguageEnglish
Peer reviewedYes
NRC numberALTMAN1991A
NPARC number9360703
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Record identifier492586f3-8239-4888-8931-1dad798ddbb7
Record created2009-07-10
Record modified2023-01-05
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