Language selection

Government of Canada / Gouvernement du Canada

Search

Multi‐omics atlas of combinatorial abiotic stress responses in wheat

From National Research Council Canada

Download
  1. (PDF, 3.6 MiB)
DOIResolve DOI: https://doi.org/10.1111/tpj.16332
AuthorSearch for: 1, 2; Search for: 1; Search for: 3ORCID identifier: https://orcid.org/0000-0002-9345-9640; Search for: 3; Search for: 3; Search for: 1; Search for: 4; Search for: 1; Search for: 1; Search for: 5; Search for: 5; Search for: 1; Search for: 5ORCID identifier: https://orcid.org/0000-0001-5551-7232; Search for: 5ORCID identifier: https://orcid.org/0000-0002-2173-0876; Search for: 1ORCID identifier: https://orcid.org/0000-0002-7213-1590
Affiliation
  1. National Research Council of Canada. Aquatic and Crop Resource Development
  2. Summerland Research and Development Centre Agriculture and Agri‐Food Canada
  3. Saskatoon Research and Development Centre Agriculture and Agri‐Food Canada
  4. Department of Biological Sciences University of Calgary
  5. Department of Cell and Systems Biology University of Toronto
FunderSearch for: Natural Sciences and Engineering Research Council of Canada
FormatText, Article
Subjectwheat (Triticum aestivumL.); abiotic stress; multi-omics; multi-environmental stresses; RNAseq; physiological traits; transcription factors; Ethylene-responsive element binding factor-associ-ated amphiphilic repression motif
Abstract
Field-grown crops rarely experience growth conditions in which yield can be maximized. Environmental stresses occur in combination, with advancements in crop tolerance further complicated by its polygenic nature. Strategic targeting of causal genes is required to meet future crop production needs. Here, we employed a systems biology approach in wheat (Triticum aestivum L.) to investigate physio-metabolic adjustments and transcriptome reprogramming involved in acclimations to heat, drought, salinity and all combinations therein. A significant shift in magnitude and complexity of plant response was evident across stress scenarios based on the agronomic losses, increased proline concentrations and 8.7-fold increase in unique differentially expressed transcripts (DETs) observed under the triple stress condition. Transcriptome data from all stress treatments were assembled into an online, open access eFP browser for visualizing gene expression during abiotic stress. Weighted gene co-expression network analysis revealed 152 hub genes of which 32% contained the ethylene-responsive element binding factor-associated amphiphilic repression (EAR) transcriptional repression motif. Cross-referencing against the 31 DETs common to all stress treatments isolated TaWRKY33 as a leading candidate for greater plant tolerance to combinatorial stresses. Integration of our findings with available literature on gene functional characterization allowed us to further suggest flexible gene combinations for future adaptive gene stacking in wheat. Our approach demonstrates the strength of robust multi-omics-based data resources for gene discovery in complex environmental conditions. Accessibility of such datasets will promote cross-validation of candidate genes across studies and aid in accelerating causal gene validation for crop resiliency.
Publication date
PublisherWiley
Licence
In
  • The Plant Journal (29 May 2023).
LanguageEnglish
Peer reviewedYes
Export citationExport as RIS
Report a correctionReport a correction (opens in a new tab)
Record identifier46b45728-167a-4ae7-8938-26447d309ed4
Record created2023-07-31
Record modified2023-07-31
Date modified: