| DOI | Resolve DOI: https://doi.org/10.1080/15321810701603443 |
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| Author | Search for: Nielsen, K.; Search for: Yu, W.; Search for: Lin, M.; Search for: Davis, S.; Search for: Elmgren, C.; Search for: MacKenzie, Colin1; Search for: Tanha, Jamshid1; Search for: Li, S.1; Search for: Dubuc, G.1; Search for: Brown, E.; Search for: Keleta, L.; Search for: Pasick, J. |
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| Affiliation | - National Research Council Canada. NRC Institute for Biological Sciences
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| Format | Text, Article |
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| Subject | Lateral flow technology, Avian influenza virus, Chicken antibody |
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| Abstract | A rapid and effective lateral flow assay (LFA) for detection of avian influenza virus (AIV) was developed. For antigen capture, the assay used monoclonal antibody specific for a conserved nuclear protein (NP) epitope, immobilized on a cellulose acetate matrix, in conjunction with a second NP monoclonal antibody chemically linked to either coloured latex beads or colloidal gold particles contained in a sample pad for detection. Virus sample added to the sample pad flowed into the trapping antibody to form a visible band as well as a second, control band further along the acetate strip. The control band consisted of recombinant protein A/G, also immobilized on the matrix. A second LFA for detection of chicken antibody to AIV was developed where NP antigen was immobilized on the matrix with recombinant protein A/G immobilized as a control band. Latex beads or colloidal gold particles to which monoclonal anti-chicken antibody was attached, were used as the indicator system. |
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| Publication date | 2007-09-27 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | NIELSEN2007 |
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| NPARC number | 9379036 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 3f19441b-c629-46bd-bccc-0d34eba0f416 |
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| Record created | 2009-07-10 |
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| Record modified | 2020-05-10 |
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