Language selection

Government of Canada / Gouvernement du Canada

Search

Single-domain antibody-nanoparticles : Promising architecturres for increased Staphylococcus aureus detection specificity and sensitivity

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1021/bc900332r
AuthorSearch for: 1; Search for: 2; Search for: 1; Search for: 3; Search for: 2; Search for: 1; Search for: 1; Search for: 1
Affiliation
  1. National Research Council of Canada. NRC Institute for Biological Sciences
  2. National Research Council of Canada. NRC Steacie Institute for Molecular Sciences
  3. National Research Council of Canada. NRC Institute for Microstructural Sciences
FormatText, Article
Subjectnanoparticles; biomolecules; antibody-nanoparticle; nanoconjugates; biodiagnostics; superparamagnetic
Abstract
Because antibodies are highly target-specific and nanoparticles possess diverse, material-dependent properties that can be exploited in order to label and potentially identify biomolecules, the development of antibody-nanoparticle conjugates (nanoconjugates) has huge potential in biodiagnostics. Here, we describe a novel superparamagnetic nanoconjugate, one whose recognition component is a single-domain antibody. It is highly active toward its target Staphylococcus aureus, displays long shelf life, lacks cross-reactivity inherent to traditional homologue whole antibodies, and captures a few dozen S. aureus cells in a mixed cell population with ∼100% efficiency and specificity. We ascribe the excellent performance of our nanoconjugate to its single-domain antibody component and recommend it as a general purpose recognition element.
Publication date
In
  • Bioconjugate Chemistry 20, no. 10 (2009): 1966–1974.
LanguageEnglish
Peer reviewedYes
NPARC number15295296
Export citationExport as RIS
Report a correctionReport a correction (opens in a new tab)
Record identifier39a496cb-f0ce-409c-b13a-56c3ebf1eb03
Record created2010-05-17
Record modified2020-04-16
Date modified: