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Thermodynamic studies on synthetic lac operator core

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1016/0006-291X(78)91180-4
AuthorSearch for: ; Search for: ; Search for: 1
Affiliation
  1. National Research Council Canada. NRC Institute for Biological Sciences
FormatText, Article
Abstract
The nine base pairs long central region of the lac operator gene forms a stable double helix. A comparison of melting temperatures with other biologically useful oligonucleotides indicates the importance of specific base sequence. Binding constants measured with ethidium bromide (1.7 × 10^5 M^−1), tyrosine (4.0 × 10^3 M^−1), and glutamine (1.5 × 10^3 M^−1), are interpreted in terms of the involvement of a relatively small number of amino acids in the lac operator-repressor interaction.
Publication date
PublisherElsevier
In
  • Biochemical and Biophysical Research Communications 85, no. 4: 1560–1567.
LanguageEnglish
Peer reviewedYes
NRC numberPATEL1978C
NPARC number9381373
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Record identifier3914a459-0323-4bc2-8950-c4f5807dc531
Record created2009-07-10
Record modified2020-03-13
Date modified: