| DOI | Resolve DOI: https://doi.org/10.1016/j.aquaculture.2004.02.001 |
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| Author | Search for: Perez-Casanova, J. C.1; Search for: Murray, H. M.2; Search for: Gallant, J. W.3; Search for: Ross, N. W.4; Search for: Douglas, S. E.3; Search for: Johnson, S. C.2 |
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| Affiliation | - National Research Council of Canada
- National Research Council of Canada. NRC Institute for Marine Biosciences
- National Research Council of Canada. Human Health Therapeutics
- National Research Council of Canada. Industrial Research Assistance Program
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| Format | Text, Article |
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| Subject | bile salt-activated lipase; fish larvae; gene expression; haddock; pancreas |
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| Abstract | In the present work, we report the characterization of the partial cDNA sequence of a bile salt-activated lipase (BAL) cDNA from haddock. The predicted polypeptide encoded by the cDNA sequence contains the bile salt-binding site characteristic of all BALs at amino acid positions 36–45, and the lipid-binding site thus far only reported in fish BALs starting at position 345. Other features of BAL are also present including: the active site serine motif at positions 111–117, the catalytic triad formed by the residues S114, D239 and H358, and an N-glycosylation site at position 107. The relative levels of BAL gene expression were determined in haddock larvae during ontogeny by reverse transcription–polymerase chain reaction (RT–PCR) with the earliest detectable transcript levels identified at hatch. Using in situ hybridization, the BAL transcripts were localized consistently in the pancreas of haddock larvae from mouth opening until 401 degree days (DD). Using biochemical techniques, the specific activity of BAL was found to decline significantly over time. Our results also suggest that haddock larvae are capable of digesting lipids at the time of mouth opening. |
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| Publication date | 2004-06-01 |
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| Terms of use | - Crown Copyright D 2004 Published by Elsevier B.V. All rights reserved.
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | NRCC 42432
1329 |
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| NPARC number | 3538113 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 38f8d279-64ae-46c3-991f-db091b1c20f4 |
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| Record created | 2009-03-01 |
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| Record modified | 2020-04-17 |
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