DOI | Resolve DOI: https://doi.org/10.1016/j.chroma.2008.08.112 |
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Author | Search for: Gao, Jin-Ming1; Search for: Kamnaing, Pierre1; Search for: Kiyota, Taira1; Search for: Watchueng, Jean1; Search for: Kubo, Takahiro1; Search for: Jarussophonb, Suwatchai1; Search for: Konishi, Yasuo1 |
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Affiliation | - National Research Council of Canada. NRC Biotechnology Research Institute
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Format | Text, Article |
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Subject | high-performance displacement chromatography; HPDC; palmatine; dl-Tetrahydropalmatine; protoberberine alkaloid; Enantia chlorantha |
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Abstract | Palmatine and its reduced form, dl-tetrahydropalmatine are a group of isoquinoline alkaloids that have been reported to display a variety of biological and pharmacological activities. Both drugs are hydrophilic and are difficult to be purified by conventional purification methods of natural products. A high-performance displacement chromatography (HPDC) method successfully purified palmatine and its semi-synthetic derivative dl-tetrahydropalmatine from crude extract of the African medicinal plant Enantia chlorantha. The crude extract from the root bark of E. chlorantha was fractionated on an analytical reversed-phase C18 column by using 0.1% trifluoroacetic acid (TFA) or acetic acid/H2O as a carrier and cetylpyridinium trifluoroacetate (or acetate) (1.9 mg/mL) in 0.1% TFA (or acetic acid)/H2O as a displacer. Palmatine was quantitatively purified at >98% purity in the fully developed displacement mode. dl-Tetrahydropalmatine was semi-synthesized by NaBH4 reduction from crude palmatine and directly purified by HPDC. Both palmatine and dl-tetrahydropalmatine were identified by high-resolution electrospray tandem mass spectrometry, 1H NMR and 13C NMR. This is the first report of one-step HPDC purification of natural and semi-synthetic products from a complex crude extract. |
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Publication date | 2008-02-05 |
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In | |
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Language | English |
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Peer reviewed | Yes |
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NRC number | NRCC 47520 |
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NPARC number | 21268255 |
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Export citation | Export as RIS |
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Report a correction | Report a correction (opens in a new tab) |
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Record identifier | 3859ddb4-cdd2-4365-9648-cf57f4278c7c |
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Record created | 2013-06-10 |
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Record modified | 2020-04-15 |
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