Comparison of the 5.8s rDNA and internal transcribed spacer sequences of isolates of Leptosphaeria maculans from different pathogenicity groups

DOI	Resolve DOI: https://doi.org/10.1007/BF00312640
Author	Search for: Morales, Victor M.¹; Search for: Pelcher, Lawrence E.¹; Search for: Taylor, Janet L.¹
Affiliation	National Research Council Canada. NRC Plant Biotechnology Institute
Format	Text, Article
Subject	blackleg; phytopathogen; ribosomal RNA; Leptosphaeria
Abstract	The regions coding for the 5.8s rRNA and the flanking internal transcribed spacers (ITS1 and ITS2) from nine isolates of the blackleg pathogen Leptosphaeria maculans and one isolate of Sclerotinia sclerotiorum were amplified by the polymerase chain reaction and sequenced. Five of the L. maculans isolates were highly virulent to Brassica plants, two were weakly virulent and two were isolated from the cruciferous weed Thlaspi arvense. The 5.8s DNA sequences of all L. maculans isolates were identical. However, there were major differences in both ITS1 and ITS2 sequences that correlated with the pathogenicity grouping. Phylogenetic analysis of the ITS sequences by both parsimony and maximum-likelihood methods indicated that each pathogenicity group was statistically different from each other with the weaklyvirulent isolates being more closely related to the Thlaspi than to the highly-virulent isolates. The relationships of L. maculans to other fungi, based on a comparison of the 5.8s rDNA sequences, are discussed.
Date published	1993
Publisher	Springer Nature
In	Current Genetics 23, no. 5-6 (1993): 490–495.
Language	English
Peer reviewed	Yes
Identifier	BF00312640 NRCC-35120
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Record identifier	3510ebc4-1ac0-4662-9f43-1177e7eb4901
Record created	2023-11-06
Record modified	2023-11-06

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