| Download | - View final version: Assessment of brain‐derived extracellular vesicle enrichment for blood biomarker analysis in age‐related neurodegenerative diseases: an international overview (PDF, 2.5 MiB)
- View supplementary information: Assessment of brain‐derived extracellular vesicle enrichment for blood biomarker analysis in age‐related neurodegenerative diseases: an international overview (ZIP, 1.6 MiB)
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| DOI | Resolve DOI: https://doi.org/10.1002/alz.13823 |
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| Author | Search for: Badhwar, AmanPreet; Search for: Hirschberg, Yael; Search for: Valle‐Tamayo, Natalia; Search for: Iulita, M. Florencia; Search for: Udeh‐Momoh, Chinedu T.; Search for: Matton, Anna; Search for: Tarawneh, Rawan M.; Search for: Rissman, Robert A.; Search for: Ledreux, Aurélie; Search for: Winston, Charisse N.; Search for: Haqqani, Arsalan S.1 |
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| Affiliation | - National Research Council Canada. Human Health Therapeutics
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| Funder | Search for: National Institutes of Health; Search for: National Institute on Aging; Search for: Flemish Institute for Technological Research |
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| Format | Text, Article |
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| Subject | age-related neurodegenerative diseases; Alzheimer's disease; biofluid based biomarkers; blood; brain-derived extracellular vesicles; exosomes; immunoprecipitation-based enrichment; microvesicles; novel biomarkers; protocol variability |
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| Abstract | INTRODUCTION: Brain-derived extracellular vesicles (BEVs) in blood allows for minimally-invasive investigations of central nervous system (CNS) -specific markers of age-related neurodegenerative diseases (NDDs). Polymer-based EV- and immunoprecipitation (IP)-based BEV-enrichment protocols from blood have gained popularity. We systematically investigated protocol consistency across studies, and determined CNS-specificity of proteins associated with these protocols. METHODS: NDD articles investigating BEVs in blood using polymer-based and/or IP-based BEV enrichment protocols were systematically identified, and protocols compared. Proteins used for BEV-enrichment and/or post-enrichment were assessed for CNS- and brain-cell-type-specificity, extracellular domains (ECD+), and presence in EV-databases. RESULTS: A total of 82.1% of studies used polymer-based (ExoQuick) EV-enrichment, and 92.3% used L1CAM for IP-based BEV-enrichment. Centrifugation times differed across studies. A total of 26.8% of 82 proteins systematically identified were CNS-specific: 50% ECD+, 77.3% were listed in EV-databases. CONCLUSIONS: We identified protocol steps requiring standardization, and recommend additional CNS-specific proteins that can be used for BEV-enrichment or as BEV-biomarkers. Highlights. Across NDDs, we identified protocols commonly used for EV/BEV enrichment from blood. We identified protocol steps showing variability that require harmonization. We assessed CNS-specificity of proteins used for BEV-enrichment or found in BEV cargo. CNS-specific EV proteins with ECD+ or without were identified. We recommend evaluation of blood-BEV enrichment using these additional ECD+ proteins. |
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| Publication date | 2024-06-12 |
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| Publisher | Wiley |
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| Licence | |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 3437b339-b514-46a2-bd46-1e0c593cd0c4 |
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| Record created | 2024-09-09 |
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| Record modified | 2024-09-09 |
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