| DOI | Resolve DOI: https://doi.org/10.1255/ejms.399 |
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| Author | Search for: Li, Jianjun; Search for: Tremblay, Tammy-Lynn; Search for: Thibault, Pierre; Search for: Wang, Christa1; Search for: Attiya, Said; Search for: Harrison, D2 |
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| Affiliation | - National Research Council of Canada. NRC Institute for Information Technology
- National Research Council of Canada
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| Format | Text, Article |
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| Subject | microfabricated device; capillary electrophoresis; nanoelectrospray; tandem mass spectrometry; protein digests; Neisseria meningitidis; proteomics |
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| Abstract | An integrated microsystem providing rapid analyses of trace-level tryptic digests for proteomics application is presented. This modular microsystem includes an autosampler and a microfabricated device comprising a sample introduction port and an array of separation channels together with a low dead-volume facility enabling an interface to nanoelectrospray mass spectrometry. Sequential injection and separation of peptide standards and tryptic digests was achieved with a throughput of up to 30 samples per hour with less than 3% sample carryover. Replicate injections of peptide mixtures indicated that reproducibility of migration time was typically better than 2.3% relative standard deviation (RSD), whereas RSD values of 3.7–11.8% were observed on peak height. Mass spectral detection of sub-micromolar protein digests (< 7 fmol per injection) was achieved using a quadrupole/time-of-flight instrument in less than 2 min per sample with peak widths of 1.8–7.0 s. The analytical potential of this integrated device for the identification of gel-isolated proteins from Neisseria meningitidis immunotype L3 has been demonstrated using both peptide mass-fingerprint database searching and on-line tandem mass spectrometry. |
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| Publication date | 2001-04-01 |
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| Publisher | SAGE |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | LI2001 |
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| NPARC number | 9378471 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 31fc7eea-c35d-404e-9061-f1f8f3e1e94c |
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| Record created | 2009-07-10 |
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| Record modified | 2020-03-27 |
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