| DOI | Resolve DOI: https://doi.org/10.1139/m72-193 |
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| Author | Search for: Blakley, E. R.1 |
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| Affiliation | - National Research Council of Canada. Prairie Regional Laboratory
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| Format | Text, Article |
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| Abstract | An unidentified bacterium degrades p-hydroxyphenylacetic acid by a pathway which involves homogentisic acid as an intermediate. Extracts of cells grown on p-hydroxyphenylacetic acid contain an enzyme that converts p-hydroxyphenylacetic acid to homogentisic acid. The enzyme has been partially purified by ammonium sulfate fractionation and some of its properties examined. The complete oxidation of 1 mole of p-hydroxyphenylacetic acid requires 1 mole of either NADH or NADPH, and 1 mole of oxygen, and results in the production of 1 mole of homogentisic acid. The enzyme preparation has a high specificity for p-hydroxyphenylacetic acid, but has about 25% activity with p-hydroxyphenylpyruvic acid. |
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| Publication date | 1972-08-01 |
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| Publisher | Canadian Science Publishing |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| Identifier | NRCC-12525 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 314fdf33-6d21-449a-8481-794910c8baf6 |
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| Record created | 2023-09-11 |
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| Record modified | 2023-09-11 |
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