Promoter sequences from two different Brassica napus tapetal oleosin-like genes direct tapetal expression of ß-glucuronidase in transgenic Brassica plants

From National Research Council Canada

DOI	Resolve DOI: https://doi.org/10.1023/A:1005840824926
Author	Search for: Ping Hong, Hai¹; Search for: Ross, Joanne H.E.; Search for: Gerster, Jean L.; Search for: Rigas, Stamatis; Search for: Datla, Raju S.S.¹; Search for: Hatzopoulos, Polydefkis; Search for: Scoles, Graham; Search for: Keller, Wilf¹; Search for: Murphy, Denis J.; Search for: Robert, Laurian S.
Affiliation	National Research Council of Canada. NRC Plant Biotechnology Institute
Format	Text, Article
Subject	anther; Brassica; ß-glucuronidase; oleosin; promoter; tapetum
Abstract	To investigate the sequences responsible for the regulated expression of tapetal-specific oleosin-like genes, ca. 2 kb of the 5′-upstream regions from two divergent genes, OlnB;4 and OlnB;13, were isolated, sequenced and fused to the reporter gene β-glucuronidase for study in transgenic Brassica napus plants. Although the proteins encoded by these two genes are highly divergent, except for the conserved oleosin-like domain, the first 250 bp of their 5′-upstream regions was 86% identical, including a region of 150 bp upstream from the TATA box. Analysis of 42 independent transformants by histochemical and fluorometric methods showed that both promoters directed tapetal-specific expression that peaked at the 4 mm flower bud stage.
Publication date	1997-06
Publisher	Springer Link
In	Plant Molecular Biology 34, no. 3 (June 1997): 549–555.
Language	English
Peer reviewed	Yes
Identifier	138684
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Record identifier	30c707ce-74ae-4676-920c-52ca6ab5c63e
Record created	2023-11-01
Record modified	2023-11-01

Date modified:: 2025-11-10