| DOI | Resolve DOI: https://doi.org/10.1166/jns.2009.1007 |
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| Author | Search for: Pierson, Laure-Anne; Search for: Lalanne, Muriel; Search for: Brambilla, Davide; Search for: Marsaud, Véronique; Search for: Nicolas, Valérie; Search for: Ball, Rita; Search for: Stanimirovic, Danica1; Search for: Couvreur, Patrick; Search for: Andrieux, Karine |
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| Affiliation | - National Research Council of Canada
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| Format | Text, Article |
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| Subject | brain endothelial cells; didanosine; glycerolipidic prodrug; hiv; PEGylated nanoparticles |
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| Abstract | PEGylated biodegradable nanoparticles based on poly(alkyl cyanoacrylate) polymers have been investigated to encapsulate didanosine prodrugs as an approach to treat HIV-1 associated dementia. To achieve this goal, an amphiphilic poly[(poly(ethylene glycol) cyanoacrylate)-co-(hexadecyl cyanoacrylate)] (PEGCA-PHDCA) copolymer has been synthesized and two different didanosine prodrugs have been used; the dipalmitoyl glycerol didanosine (ProddINP) and the dipalmitoyl glycerol phosphate didanosine (ProddIP), only differ by the presence or not of a phosphate group in their chemical structure. By means of differential scanning calorimetry (DSC), the capacity of PEGCA-PHDCA copolymer to incorporate ProddINP and ProddIP prodrugs was investigated. PEGCA-PHDCA nanoparticles were then prepared via an optimised solvent emulsion/evaporation technique using the incorporation ratios determined by DSC. Finally, confocal laser scanning microscopy experiments have shown these prodrugs-loaded nanoparticles were uptaken by rat immortalised brain endothelial cells (SV-ARBEC). These first results appeared promising for further in vitro and in vivo evaluation of these nanoparticles. |
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| Publication date | 2009-12 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 21275165 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 30327620-6fc6-4b8e-8ef5-50dba99bb491 |
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| Record created | 2015-05-21 |
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| Record modified | 2020-04-16 |
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