Production of triterpenoid sapogenins in hairy root cultures of Silene vulgaris

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  1. National Research Council Canada. Aquatic and Crop Resource Development
FormatText
TypeArticle
Journal titleNatural Product Communications
ISSN1934-578X
1555-9475
Volume10
Issue11
Pages1919–1922
Abstract
Silene vulgaris (Moench) Garcke (Caryophyllaceae) is widely distributed in North America and contains bioactive oleanane-type saponins. In order to investigate in vitro production of triterpenoid saponins, hairy root cultures of S. vulgaris were established by infecting leaf explants with five strains of Agrobacterium rhizogenes (LBA9402, R1000, A4, 13333, and 15834). The A. rhizogenes strain LBA9402 had an infection of 100% frequency and induced the most hairy roots per plant. Methyl jasmonate (MeJA)-induced changes in triterpenoid saponins in S. vulgaris hairy roots were analyzed. Accumulation of segetalic acid and gypsogenic acid after MeJA treatment was 5-and 2-fold higher, respectively, than that of control root. We suggest that hairy root cultures of S. vulgaris could be an important alternative approach to the production of saponins.
Publication date
PublisherNatural Product Communications Inc.
LanguageEnglish
Peer reviewedYes
NRC numberNRC-ACRD-54676
NPARC number23000698
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Record identifier300ee09b-a12f-4d4b-8e13-768a60d2b461
Record created2016-08-25
Record modified2019-07-19
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