| DOI | Resolve DOI: https://doi.org/10.1101/pdb.prot4980 |
|---|
| Author | Search for: Tom, Roseanne1; Search for: Bisson, Louis1; Search for: Durocher, Yves1 |
|---|
| Affiliation | - National Research Council of Canada. NRC Biotechnology Research Institute
|
|---|
| Format | Text, Article |
|---|
| Abstract | Fast and efficient production of recombinant proteins (r-proteins) remains a major challenge for the academic and biopharmaceutical communities. Such proteins often need to be as pure as possible before any characterization study can begin. Although many types of protein tag are available, histidine is the most popular. Although small-scale immobilized metal-affinity column (IMAC) purification of such proteins (e.g., <500 mL of culture medium) can easily be achieved using gravity chromatography columns, larger volumes can be processed with the aid of automated chromatography systems. This protocol describes an IMAC purification technique for secreted proteins using a cobalt-loaded resin. Preliminary small-scale trials using this technique can be used to determine the production scale that will be needed to provide enough pure material for a given study. |
|---|
| Publication date | 2008-03 |
|---|
| In | |
|---|
| Language | English |
|---|
| Peer reviewed | Yes |
|---|
| NRC number | NRCC 53207 |
|---|
| NPARC number | 21268300 |
|---|
| Export citation | Export as RIS |
|---|
| Report a correction | Report a correction (opens in a new tab) |
|---|
| Record identifier | 2fa31bbc-943e-4eef-8f14-de962746ff45 |
|---|
| Record created | 2013-06-17 |
|---|
| Record modified | 2020-04-15 |
|---|
