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Picomolar analysis of proteins using electrophoretically mediated microanalysis and capillary electrophoresis with laser-induced fluorescence detection

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1021/ac980360t
AuthorSearch for: 1; Search for: 1ORCID identifier: https://orcid.org/0000-0002-0665-7041; Search for: 1; Search for: 1; Search for: 1
Affiliation
  1. Department of Chemistry, University of Alberta
FormatText, Article
Abstract
We report a method for the analysis of picomolar concentration proteins using electrophoretically mediated microanalysis (EMMA) to label proteins on-column with a fluorogenic reagent. Labeling is followed by capillary zone electrophoresis separation and postcolumn detection based on laser-induced fluorescence. The method provides a concentration detection limit (3σ) of 3 × 10⁻¹³ M for conalbumin. The method provides separation efficiency of 300 000 theoretical plates. Protein extract from a human colon adenocarcinoma cell line generated a dozen major components and many minor components in a 12-min separation; the protein extract from 2.5 cells was used for this analysis. When compared to UV absorbance detection, the EMMA method provides 7 000 000-fold improvement in detection limit.
Publication date
PublisherAmerican Chemical Society
In
  • Analytical Chemistry 70, no. 21 (1 November 1998): 4546–4548.
LanguageEnglish
Peer reviewedYes
NRC publication
This is a non-NRC publication

"Non-NRC publications" are publications authored by NRC employees prior to their employment by NRC.

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Record identifier2d3cc7d9-9279-42b3-a090-941275f5294a
Record created2024-07-11
Record modified2024-07-11
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