| Download | - View final version: Collagen tubular airway‐on‐chip for extended epithelial culture and investigation of ventilation dynamics (PDF, 6.4 MiB)
- View supplementary information: Collagen tubular airway‐on‐chip for extended epithelial culture and investigation of ventilation dynamics (PDF, 7.7 MiB)
|
|---|
| DOI | Resolve DOI: https://doi.org/10.1002/smll.202309270 |
|---|
| Author | Search for: Gao, Wuyang1; Search for: Kanagarajah, Kayshani R.1; Search for: Graham, Emma2, 3; Search for: Soon, Kayla4; Search for: Veres, Teodor4, 1; Search for: Moraes, Theo J.1; Search for: Bear, Christine E.1; Search for: Veldhuizen, Ruud A.2, 3; Search for: Wong, Amy P.1; Search for: Günther, Axel1ORCID identifier: https://orcid.org/0000-0002-0592-2261 |
|---|
| Affiliation | - University of Toronto
- University of Western Ontario
- London Health Sciences Centre
- National Research Council Canada. Medical Devices
|
|---|
| Funder | Search for: Natural Sciences and Engineering Research Council of Canada; Search for: National Research Council Canada; Search for: University of Toronto |
|---|
| Format | Text, Article |
|---|
| Subject | air-liquid interface culture; airway on a chip; collagen tube; collapsible tube; ventilator-induced lung injury |
|---|
| Abstract | The lower respiratory tract is a hierarchical network of compliant tubular structures that are made from extracellular matrix proteins with a wall lined by an epithelium. While microfluidic airway-on-a-chip models incorporate the effects of shear and stretch on the epithelium, week-long air-liquid-interface culture at physiological shear stresses, the circular cross-section, and compliance of native airway walls have yet to be recapitulated. To overcome these limitations, a collagen tube-based airway model is presented. The lumen is lined with a confluent epithelium during two-week continuous perfusion with warm, humid air while presenting culture medium from the outside and compensating for evaporation. The model recapitulates human small airways in extracellular matrix composition and mechanical microenvironment, allowing for the first time dynamic studies of elastocapillary phenomena associated with regular breathing and mechanical ventilation, as well as their impacts on the epithelium. A case study reveales increasing damage to the epithelium during repetitive collapse and reopening cycles as opposed to overdistension, suggesting expiratory flow resistance to reduce atelectasis. The model is expected to promote systematic comparisons between different clinically used ventilation strategies and, more broadly, to enhance human organ-on-a-chip platforms for a variety of tubular tissues. |
|---|
| Publication date | 2024-03-03 |
|---|
| Publisher | Wiley |
|---|
| Licence | |
|---|
| In | |
|---|
| Language | English |
|---|
| Peer reviewed | Yes |
|---|
| Export citation | Export as RIS |
|---|
| Report a correction | Report a correction (opens in a new tab) |
|---|
| Record identifier | 26fd4d0c-a621-4703-a9ae-474eaafda889 |
|---|
| Record created | 2024-09-11 |
|---|
| Record modified | 2025-11-03 |
|---|
