Production of lentiviral vectors encoding recombinant factor VIII expression in serum-free suspension cultures

DOI	Resolve DOI: https://doi.org/10.1590/S1516-89132015060367
Author	Search for: Caron, Angelo Luis; Search for: Picanço-Castro, Virgínia; Search for: Ansorge, Sven¹; Search for: Covas, Dimas Tadeu; Search for: Kamen, Amine; Search for: Swiech, Kamilla
Name affiliation	National Research Council of Canada. Human Health Therapeutics
Format	Text, Article
Abstract	Lentiviral vector-mediated gene transfer offers several advantages over other gene delivery vectors when considering gene and cell therapy applications. However, using these therapies in clinical applications involves large-scale vector production in an efficient and cost-effective manner. Here we describe a high yield production of a lentivirus encoding recombinant factor VIII in a scalable and GMP-compliant culture system, based on serum free suspension cultures and transient transfection with an inexpensive reagent, polyethylenimine (PEI), reaching a total viral yield of 2.48x108 particles.
Publication date	2015-12
In	Brazilian Archives of Biology and Technology 58, no. 6 (December 2015): 923–928.
Language	English
Peer reviewed	Yes
NPARC number	21277344
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Record identifier	26e043c9-1916-42c4-9272-2352a3ec2215
Record created	2016-03-09
Record modified	2020-04-22