Production of lentiviral vectors encoding recombinant factor VIII expression in serum-free suspension cultures

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1590/S1516-89132015060367
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Name affiliation
  1. National Research Council of Canada. Human Health Therapeutics
FormatText, Article
Journal titleBrazilian Archives of Biology and Technology
ISSN1516-8913
Volume58
Issue6
Pages923–928
Abstract
Lentiviral vector-mediated gene transfer offers several advantages over other gene delivery vectors when considering gene and cell therapy applications. However, using these therapies in clinical applications involves large-scale vector production in an efficient and cost-effective manner. Here we describe a high yield production of a lentivirus encoding recombinant factor VIII in a scalable and GMP-compliant culture system, based on serum free suspension cultures and transient transfection with an inexpensive reagent, polyethylenimine (PEI), reaching a total viral yield of 2.48x108 particles.
Publication date
PublisherBrazilian Archives of Biology and Technology
LanguageEnglish
Peer reviewedYes
NPARC number21277344
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Record identifier26e043c9-1916-42c4-9272-2352a3ec2215
Record created2016-03-09
Record modified2020-04-22
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