DOI | Resolve DOI: https://doi.org/10.1590/S1516-89132015060367 |
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Author | Search for: Caron, Angelo Luis; Search for: Picanço-Castro, Virgínia; Search for: Ansorge, Sven1 ; Search for: Covas, Dimas Tadeu; Search for: Kamen, Amine; Search for: Swiech, Kamilla |
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Name affiliation | - National Research Council Canada. Human Health Therapeutics
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Format | Text |
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Type | Article |
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Journal title | Brazilian Archives of Biology and Technology |
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ISSN | 1516-8913 |
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Volume | 58 |
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Issue | 6 |
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Pages | 923–928; # of pages: 6 |
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Abstract | Lentiviral vector-mediated gene transfer offers several advantages over other gene delivery vectors when considering gene and cell therapy applications. However, using these therapies in clinical applications involves large-scale vector production in an efficient and cost-effective manner. Here we describe a high yield production of a lentivirus encoding recombinant factor VIII in a scalable and GMP-compliant culture system, based on serum free suspension cultures and transient transfection with an inexpensive reagent, polyethylenimine (PEI), reaching a total viral yield of 2.48x108 particles. |
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Publication date | 2015-12 |
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Publisher | Brazilian Archives of Biology and Technology |
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Language | English |
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Peer reviewed | Yes |
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NPARC number | 21277344 |
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Export citation | Export as RIS |
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Report a correction | Report a correction |
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Record identifier | 26e043c9-1916-42c4-9272-2352a3ec2215 | Record created | 2016-03-09 |
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Record modified | 2016-05-09 |
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