| DOI | Resolve DOI: https://doi.org/10.1590/S1516-89132015060367 |
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| Author | Search for: Caron, Angelo Luis; Search for: Picanço-Castro, Virgínia; Search for: Ansorge, Sven1; Search for: Covas, Dimas Tadeu; Search for: Kamen, Amine; Search for: Swiech, Kamilla |
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| Name affiliation | - National Research Council of Canada. Human Health Therapeutics
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| Format | Text, Article |
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| Journal title | Brazilian Archives of Biology and Technology |
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| ISSN | 1516-8913 |
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| Volume | 58 |
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| Issue | 6 |
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| Pages | 923–928 |
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| Abstract | Lentiviral vector-mediated gene transfer offers several advantages over other gene delivery vectors when considering gene and cell therapy applications. However, using these therapies in clinical applications involves large-scale vector production in an efficient and cost-effective manner. Here we describe a high yield production of a lentivirus encoding recombinant factor VIII in a scalable and GMP-compliant culture system, based on serum free suspension cultures and transient transfection with an inexpensive reagent, polyethylenimine (PEI), reaching a total viral yield of 2.48x108 particles. |
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| Publication date | 2015-12 |
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| Publisher | Brazilian Archives of Biology and Technology |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 21277344 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction |
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Record identifier | 26e043c9-1916-42c4-9272-2352a3ec2215 | | Record created | 2016-03-09 |
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| Record modified | 2020-04-22 |
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