| DOI | Resolve DOI: https://doi.org/10.1016/j.atherosclerosis.2015.12.032 |
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| Author | Search for: Schweitzer, Morris; Search for: Makhoul, Sandra; Search for: Paliouras, Miltiadis; Search for: Beitel, Lenore K.; Search for: Gottlieb, Bruce; Search for: Trifiro, Mark; Search for: Chowdhury, Shafinaz F.1; Search for: Zaman, Naif M.1; Search for: Wang, Edwin1; Search for: Davis, Harry; Search for: Chalifour, Lorraine E. |
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| Affiliation | - National Research Council of Canada. Human Health Therapeutics
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| Format | Text, Article |
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| Subject | cholesterol; cholesterol/absorption; cholesterol/cell and tissue; drug therapy/hypolipidemic drugs; genetics; proteomics |
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| Abstract | Background - Strategies to reduce LDL-cholesterol involve reductions in cholesterol synthesis or absorption. We identified a familial hypercholesterolemia patient with an exceptional response to the cholesterol absorption inhibitor, ezetimibe. Niemann-Pick C 1-like 1 (NPC1L1) is the molecular target of ezetimibe. Methods and results - Sequencing identified nucleotide changes predicted to change amino acids 52 (L52P), 300 (I300T) and 489 (S489G) in exceptional NPC1L1. In silico analyses identified increased stability and cholesterol binding affinity in L52P-NPC1L1 versus WT-NPC1L1. HEK293 cells overexpressing WT-NPC1L1 or NPC1L1 harboring amino acid changes singly or in combination (Comb-NPC1L1) had reduced cholesterol uptake in Comb-NPC1L1 when ezetimibe was present. Cholesterol uptake was reduced by ezetimibe in L52P-NPC1L1, I300T-NPC1L1, but increased in S489G-NPC1L1 overexpressing cells. Immunolocalization studies found preferential plasma membrane localization of mutant NPC1L1 independent of ezetimibe. Flotillin 1 and 2 expression was reduced and binding to Comb-NPC1L1 was reduced independent of ezetimibe exposure. Proteomic analyses identified increased association with proteins that modulate intermediate filament proteins in Comb-NPC1L1 versus WT-NPC1L1 treated with ezetimibe. Conclusion - This is the first detailed analysis of the role of NPC1L1 mutations in an exceptional responder to ezetimibe. The results point to a complex set of events in which the combined mutations were shown to affect cholesterol uptake in the presence of ezetimibe. Proteomic analysis suggests that the exceptional response may also lie in the nature of interactions with cytosolic proteins. |
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| Publication date | 2015-12-24 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| Identifier | S0021915015302616 |
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| NPARC number | 21277181 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 23d17299-47db-47c2-8391-f5a1b9ba5426 |
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| Record created | 2016-01-05 |
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| Record modified | 2020-04-22 |
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