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Proteomic analysis of Candida albicans yeast and hyphal cell wall and associated proteins

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1002/pmic.200500100
AuthorSearch for: 1; Search for: 1; Search for: 1; Search for: 2; Search for: 1
Affiliation
  1. National Research Council of Canada. NRC Institute for Marine Biosciences
  2. National Research Council of Canada. NRC Biotechnology Research Institute
FormatText, Article
SubjectCandida albicans; pharmaceutical; cell wall proteins; yeast-hyphal transition
Abstract
Candida albicans is an important human pathogen that causes systemic infections, predominantly among populations with weakened immune systems. The morphological transition from the yeast to the hyphal state is one of the key factors in C. albicans pathogenesis. Owing to their location at the host -pathogen interface, the cell wall and associated proteins are of interest, especially with respect to the yeast to hyphal transition. This study entailed the proteomic analysis of differentially regulated proteins involved in this transition. The protein profiles of C. albicans DTT/SDS-extractible proteins and the cyanogen bromide (CNBr)/trypsin-extractable proteins of a cell wall -enriched fraction from yeast and hyphae were compared. In total, 107 spots were identified from the DTT/SDS-extractible cell wall-enriched fraction, corresponding to 82 unique proteins. Of these DTT/SDS-extractible proteins, 14 proteins were upregulated and 10 were downregulated in response to hyphal induction. Approximately 6-9% of total cell wall-protein-enriched fraction was found to be resistant to DTT/SDS extraction. Analysis of the DTT/SDS-resistant fraction using a CNBr/trypsin extraction resulted in the identification of 29 proteins. Of these, 17 were identified only in the hyphae, four were identified only in the yeast, and eight were identified in both the yeast and hyphae
Publication date
In
  • Proteomics 6, no. 7 (22 February 2006): 2147–2156.
LanguageEnglish
Peer reviewedYes
NRC number47505
NRCC 55551
1505
NPARC number3539691
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Record identifier217fab79-c5ac-4743-9a09-716722779f64
Record created2009-03-01
Record modified2020-04-22
Date modified: