| Author | Search for: Novakovic, Predrag; Search for: Huang, Yanyun Y.; Search for: Lockerbie, Betty; Search for: Shahriar, Farshid; Search for: Kelly, John1; Search for: Gordon, John R.; Search for: Middleton, Dorothy M.; Search for: Loewen, Matthew E.; Search for: Kidney, Beverly A.; Search for: Simko, Elemir |
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| Affiliation | - National Research Council of Canada. Human Health Therapeutics
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| Format | Text, Article |
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| Subject | adipophilin; binding protein; butyrophilin; fatty acid binding protein 3; lactadherin; long chain fatty acid coenzyme A ligase 3; membrane protein; affinity chromatography; binding affinity; enterotoxigenic Escherichia coli; F4ac fimbria; fimbria; liquid chromatography; mass spectrometry; membrane; milk fat globule membrane; polyacrylamide gel electrophoresis; protein analysis; swine; Western blotting; bacteria (microorganisms); Sus; Escherichia coli |
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| Abstract | F4ac-positive enterotoxigenic Escherichia coli (ETEC) must attach to the intestinal mucosa to cause diarrhea in piglets. Prevention of bacterial attachment to the intestinal mucosa is the most effective defense against ETEC-induced diarrhea. Porcine milk fat globule membranes (MFGM) were shown to be able to inhibit attachment of ETEC to the intestinal brush border; however, the specific components of porcine MFGM that inhibited attachment of ETEC to enterocytes were not identified. Accordingly, the purpose of this study was to identify F4ac-binding MFGM proteins by overlay Western blot and affinity chromatography. The proteome of porcine MFGM was characterized and the following F4ac-binding proteins were detected by overlay Western blot and affinity chromatography: lactadherin, butyrophilin, adipophilin, acyl-CoA synthetase 3, and fatty acid-binding protein 3. The biological function of these proteins was not investigated but it is possible that their interaction with F4ac fimbria interferes with bacterial attachment and colonization. |
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| Publication date | 2015-04 |
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| Publisher | National Centre for Biotechnology Information |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 21275759 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 20791c78-da6d-45fa-9039-9ab172168d68 |
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| Record created | 2015-07-14 |
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| Record modified | 2022-11-18 |
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