Brassinosteriod Insensitive 2 (BIN2) acts as a downstream effector of the Target of Rapamycin (TOR) signaling pathway to regulate photoautotrophic growth in Arabidopsis

From National Research Council Canada

Alternative title	BIN2 acts as a downstream effector of the TOR signaling pathway to regulate photoautorophic growth in Arabidopsis
Download	View accepted manuscript: Brassinosteriod Insensitive 2 (BIN2) acts as a downstream effector of the Target of Rapamycin (TOR) signaling pathway to regulate photoautotrophic growth in Arabidopsis (PDF, 803 KB)
DOI	Resolve DOI: https://doi.org/10.1111/nph.14118
Author	Search for: Xiong, Fangjie; Search for: Zhang, Rui; Search for: Meng, Zhigang; Search for: Deng, Kexuan; Search for: Que, Yumei; Search for: Zhuo, Fengping; Search for: Feng, Li; Search for: Guo, Sundui; Search for: Datla, Raju¹; Search for: Ren, Maozhi
Name affiliation	National Research Council of Canada. Aquatic and Crop Resource Development
Format	Text, Article
Journal title	New Phytologist
ISSN	0028-646X 1469-8137
Volume	213
Issue	1
Pages	233–249
Subject	active-site TOR inhibitors (asTORis); Arabidopsis; Brassinosteriod Insensitive 2 (BIN2); photoautotrophic growth; Ribosomal protein S6 kinase 2 (S6K2); target of rapamycin (TOR)
Abstract	•The components of the target of rapamycin (TOR) signaling pathway have been well characterized in heterotrophic organisms from yeast to humans. However, because of rapamycin insensitivity, embryonic lethality in tor null mutants and a lack of reliable ways of detecting TOR protein kinase in higher plants, the key players upstream and downstream of TOR remain largely unknown in plants. •Using engineered rapamycin-sensitive Binding Protein 12-2 (BP12-2) plants, the present study showed that combined treatment with rapamycin and active-site TOR inhibitors (asTORis) results in synergistic inhibition of TOR activity and plant growth in Arabidopsis. •Based on this system, we revealed that TOR signaling plays a crucial role in modulating the transition from heterotrophic to photoautotrophic growth in Arabidopsis. Ribosomal protein S6 kinase 2 (S6K2) was identified as a direct downstream target of TOR, and the growth of TOR-suppressed plants could be rescued by up-regulating S6K2. Systems, genetic, and biochemical analyses revealed that Brassinosteriod Insensitive 2 (BIN2) acts as a novel downstream effector of S6K2, and the phosphorylation of BIN2 depends on TOR-S6K2 signaling in Arabidopsis. •By combining pharmacological with genetic and biochemical approaches, we determined that the TOR-S6K2-BIN2 signaling pathway plays important roles in regulating the photoautotrophic growth of Arabidopsis.
Publication date	2016-08-01
Publisher	Wiley
Language	English
Peer reviewed	Yes
NRC number	NRC-ACRD-56301
NPARC number	23001928
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Record identifier	203604f7-e187-4e27-825d-5d9bf277e40e
Record created	2017-06-02
Record modified	2020-06-04

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Date modified:: 2021-01-28