| Download | - View final version: Evidence for the extended helical nature of polysaccharide epitopes. The 2.8 A resolution structure and thermodynamics of ligand binding of an antigen binding fragment specific for alpha-(2-->8)-polysialic acid (PDF, 1.5 MiB)
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| DOI | Resolve DOI: https://doi.org/10.1021/bi00020a019 |
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| Author | Search for: Evans, S.1; Search for: Sigurskjold, B. W.1; Search for: Jennings, H. J.1; Search for: Brisson, J.-R.1; Search for: To, R.1; Search for: Tse, W. C.1; Search for: Altman, Eleonora1; Search for: Frosch, M.; Search for: Weisgerber, C.1; Search for: Kratzin, H. D.; Search for: Klebert, S.; Search for: Vaesen, M.; Search for: Bitter-Suermann, D.; Search for: Rose, D. R.1; Search for: Young, N. M.1; Search for: Bundle, D. R.1 |
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| Affiliation | - National Research Council of Canada
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| Format | Text, Article |
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| Abstract | The antigen binding fragment from an IgG2a kappa murine monoclonal antibody with specificity for alpha-(2-->8)-linked sialic acid polymers has been prepared and crystallized in the absence of hapten. Crystals were grown by vapor diffusion equilibrium with 16-18% polyethylene glycol 4000 solutions. The structure was solved by molecular replacement methods and refined to a conventional R factor of 0.164 for data to 2.8 A. The binding site is observed to display a shape and distribution of charges that is complementary to that of the predicted conformation of the oligosaccharide epitope. A thermodynamic description of ligand binding has been compiled for oligosaccharides ranging in length from 9 to 41 residues, and the data for the largest ligand has been used in a novel way to estimate the size of the antigen binding site. A model of antigen binding is presented that satisfies this thermodynamic data, as well as a previously reported requirement of conformational specificity of the oligosaccharide. X-ray crystallographic and thermodynamic evidence are consistent with a binding site that accommodates at least eight sialic acid residues |
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| Publication date | 1995-05-23 |
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| Publisher | American Chemical Society |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | EVANS1995 |
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| NPARC number | 9371205 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 1df6fea1-59aa-4dbc-90d2-3463988cd415 |
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| Record created | 2009-07-10 |
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| Record modified | 2020-05-27 |
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