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Regeneration of plants from callus tissue of the forage legume Stylosanthes guianensis

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1016/0304-4211(81)90034-1
AuthorSearch for: 1; Search for: 1
Affiliation
  1. National Research Council of Canada. Prairie Regional Laboratory
FormatText, Article
Abstract
Plants were regenerated from leaflets of Stylosanthes guianensis (Aubl.) Sw. cultured in vitro. Explants from both mature and immature leaflets were induced to form callus when aseptically cultured on Murashige and Skoog salts containing vitamins as in B5 medium, 3% sucrose, 0.8% agar and three combinations of napthaleneacetic acid (NAA) and benzyladenine (BA). Regeneration of shoots was readily achieved by transferring pieces of callus, even after subculturing for 6 months, onto fresh medium containing a low NAA/BA ratio. Shoots were induced to form roots upon transfer to medium devoid of hormones.
Publication date
PublisherElsevier
In
  • Plant Science Letters 23, no. 3 (December 1981): 245–251.
LanguageEnglish
Peer reviewedYes
Identifier0304421181900341
NRCC-19589
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Record identifier1ca685a7-13b0-4af8-89ca-cbdae11ba05c
Record created2023-11-15
Record modified2023-11-15
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