| DOI | Resolve DOI: https://doi.org/10.1016/j.chroma.2007.09.031 |
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| Author | Search for: Rowe, Gerald E.1; Search for: Aomari, Hafida1; Search for: Chevaldina, Tatiana1; Search for: Lafrance, Martin1; Search for: St-Arnaud, Sylvie1 |
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| Affiliation | - National Research Council of Canada. NRC Biotechnology Research Institute
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| Format | Text, Article |
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| Subject | Acids; Adsorption; Amino Acids; analogs & derivatives; Biotechnology; chemistry; Chromatography; Chromatography,Liquid; Esters; Heat; Hydrogen Bonding; Hydrophobicity; methods; Models,Chemical; Polymers; Sepharose; Solubility; Thermodynamics; Hydrophobic interaction chromoatography; N-Acetyl amino acid methyl esters; biotechnology; Temperature |
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| Abstract | Thermodynamic analysis of hydrophobic interaction chromatography of amino acid methyl esters showed entropy-driven adsorption, consistent with solvophobic theory, except for phenyl ester on the Toyopearl resins. All esters adsorbed more strongly to the Toyopearl resins, including the polymethacrylate base matrix, than to Butyl Sepharose. Enthalpy changes were more favorable with the former, explaining the retention difference between Toyopearl Butyl and Butyl Sepharose. An enthalpy change versus heat capacity change plot showed Van derWaals interactions predominantly with the resin matrix. Literature data revealed the same effect for dansylamino acids, shown by isothermodynamic temperature analysis to adsorb more entropically than the esters. |
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| Publication date | 2007-09-18 |
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| In | |
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| Language | English |
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| NRC number | NRCC 52722 |
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| NPARC number | 12401003 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 181b34cb-8fde-4b2a-a05c-796d146f53a7 |
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| Record created | 2009-10-26 |
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| Record modified | 2020-05-10 |
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