Hepatoprotective constituents of the seeds of Combretum quadrangulare

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1248/bpb.23.1328
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FormatText, Article
Journal titleBiological and Pharmaceutical Bulletin
ISSN0918-6158
1347-5215
Volume23
Issue11
Pages1328–1332
SubjectCombretum quadrangulare; Vietnamese medicinal plant; hepatoprotective effect; triterpene glucoside; catechin; 1-O-galloyl-6-O-(4-hidroxy-3, 5-dimethoxy)benzoyl-β-D-glucose
Abstract
Hepatoprotective effect of MeOH, MeOH-H2O (1 : 1) and H2O extracts of Combretum quadrangulare seeds were examined on D-galactosamine (D-GalN)/tumor necrosis factor-α(TNF-α)-induced cell death in primary cultured mouse hepatocytes. The MeOH extract showed the strongest inhibitory effect on D-GalN/TNF-α-induced cell death (IC50, 56.4μg/ml). Moreover, the MeOH extract also significantly lowered the serum glutamic pyruvic transaminase (sGPT) level on D-GalN/lipopolysaccharide (LPS)-induced liver injury in mice. Bioguided separation of the MeOH extract led to the isolation of 38 compounds of various classes including triterpene glucosides, lignans and catechin derivatives. Among the isolated triterpene glucosides, lupane-type (1-3; IC50, 63.1, 59.8 and 76.2μM, respectively) and ursane-type (11, mixture of 12 and 14; IC50, 30.2 and 34.6μM, respectively) compounds exhibited strong hepatoprotective activity. 1-O-Galloyl-6-O-(4-hydroxy-3, 5-dimethoxy)benzoyl-β-D-glucose (26; IC50, 7.2μM), methyl gallate (28; IC50, 19.9μM), and (-)-epicatechin (31; IC50, 71.2μM) also had a potent hepatoprotective effect on D-GalN/TNF-α-induced cell death in primary cultured mouse hepatocytes.
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LanguageEnglish
Peer reviewedYes
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NPARC number21275300
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Record created2015-06-04
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