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Multiplexed quantitative proteomic profiling of cancer cells and tissues using isobaric labeling-based tags

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1007/978-1-0716-2376-3_16
AuthorSearch for: ; Search for: 1ORCID identifier: https://orcid.org/0000-0002-0665-7041; Search for:
Affiliation
  1. National Research Council Canada. Human Health Therapeutics
FormatText, Book Chapter
Abstract
Comparing cancer proteomes across many samples offers a window into cancer cell biology and may reveal new treatment options for specific subsets of cancer. Here we describe a method using tandem mass tag (TMT) technology to multiplex up to 18 samples in a single analysis, paving the way for the analysis of large cohorts of tumors, cell lines, and perturbations thereof. The procedure we describe will result in samples ready for in-depth LC-MS/MS analysis in 3–4 days.
Publication date
PublisherSpringer
In
  • Cancer Cell Biology: Methods and Protocols (22 June 2022): 211–223.
Series
  • Methods in Molecular Biology 2508.
LanguageEnglish
Peer reviewedYes
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Record identifier15a63a11-5817-4676-af1a-d965f6d95ca6
Record created2024-06-24
Record modified2024-06-24
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