Effects of the local anesthetic tetracaine on the structural and dynamic properties of lipids in model membranes: a high-pressure Fourier transform infrared study

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1021/bi00416a038
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Name affiliation
  1. National Research Council Canada
FormatText
TypeArticle
Journal titleBiochemistry
ISSN0006-2960
1520-4995
Volume27
Issue16
Pages6086–6093
Abstract
High-pressure Fourier transform infrared (FT-IR) spectroscopy was used to study the effects of a local anesthetic, tetracaine, on the structural and dynamic properties of lipids in model membranes. The model membrane systems studied were multilamellar aqueous dispersions of 1,2-dimyristoyl-m glycero-3-phosphocholine (DMPC) and 1,2-di-O-hexadecyl-sn-glycero-3-phosphocholine (DHPC) in the absence and presence of a physiological concentration of cholesterol (30 mol %). The infrared spectra were measured at 28ºC in a diamond anvil cell as a function of pressure up to 25 kbar. The results indicate that the effects of tetracaine on the structure of pure DMPC bilayers in the gel state are dependent on the state of charge of the anesthetic. The uncharged tetracaine disorders the lipid acyl chains while the charged form induces the formation of an interdigitated gel phase. The presence of cholesterol in the latter system prevents the formation of the interdigitated phase, whereas in the former system it disorders the lipid acyl chains in the gel state. Moreover, it is shown that the addition of uncharged tetracaine to interdigitated DHPC bilayers does not alter the interdigitated state of the hydrocarbon chains.
Publication date
LanguageEnglish
Peer reviewedYes
NRC number28743
NPARC number23001646
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