From plant extract to a cDNA encoding a glucosyltransferase candidate: proteomics and transcriptomics as tools to help elucidate saponin biosynthesis in Centella asiatica

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1007/978-1-4939-3393-8_5
AuthorSearch for: 1; Search for: 1; Search for: 1; Search for: 1
EditorSearch for: Arthur Germano, Fett-Neto
Name affiliation
  1. National Research Council Canada. Aquatic and Crop Resource Development
FormatText
TypeArticle
Journal titleBiotechnology of Plant Secondary Metabolism: Methods and Protocols
Series titleMethods in Molecular Biology; no. 1405
ISSN1064-3745
ISBN978-1-4939-3391-4
978-1-4939-3393-8
Pages43–48
SubjectCentella asiatica; Saponin; Glucosyltransferase; Proteomics; Transcriptomics
Abstract
Centella asiatica (L.) Urban (Apiaceae), a small annual plant that grows in India, Sri Lanka, Malaysia, and other parts of Asia, is well-known as a medicinal herb with a long history of therapeutic uses. The bioactive compounds present in C. asiatica leaves include ursane-type triterpene sapogenins and saponins—asiatic acid, madecassic acid, asiaticoside, and madecassoside. Various bioactivities have been shown for these compounds, although most of the steps in the biosynthesis of triterpene saponins, including glycosylation, remain uncharacterized at the molecular level. This chapter describes an approach that integrates partial enzyme purification, proteomics methods, and transcriptomics, with the aim of reducing the number of cDNA candidates encoding for a glucosyltransferase involved in saponin biosynthesis and facilitating the elucidation of the pathway in this medicinal plant.
Publication date
PublisherSpringer
LanguageEnglish
Peer reviewedYes
NPARC number23000697
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Record created2016-08-25
Record modified2020-03-16
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