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Oxidative metabolism and protoplast culture

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1007/BF02632035
AuthorSearch for: ; Search for: ; Search for: 1; Search for: 1
Affiliation
  1. National Research Council Canada. NRC Plant Biotechnology Institute
FormatText, Article
Subjectbarley; protoplast; oxidative stress; glutathione
Abstract
Barley leaf blade protoplasts accumulate malonaldehyde, a product of lipid peroxidation, during culture. In addition, glutathione levels fall after protoplast isolation and the proportion of glutathione in the oxidized state rises. These data indicate oxidative stress after protoplast isolation and during culture. The cause of this phenomenon is revealed by data showing that the activities of enzymes associated with antioxidative processes including glutathione reductase and ascorbate peroxidase decrease after barley protoplast isolation. In contrast, protoplasts isolated from suspension cultured cells of bromegrass and soybean exhibit little evidence for oxidative stress and increased activities of glutathione reductase and ascorbate peroxidase. We suggest that an antioxidative response is associated with mitosis and colony formation from protoplasts, as exhibited by bromegrass and soybean. Conversely, failure of an antioxidative response is associated with low viability and absence of mitosis, as in barley. Increased viability of barley leaf protoplasts cultured on feeder layer cells is correlated with increased glutathione content and higher glutathione reductase activity.
Publication date
PublisherSpringer Nature
In
  • In Vitro Cellular & Developmental Biology - Plant 29, no. 4 (October 1993): 200–206.
LanguageEnglish
Peer reviewedYes
IdentifierBF02632035
NRCC-36486
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Record identifier11c91c91-d343-4ef0-b76b-483abbca53e0
Record created2023-11-02
Record modified2023-11-02
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