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Characterization of the antigenic O-polysaccharide produced by Escherichia coli serotype O:70

DOIResolve DOI: https://doi.org/10.1016/j.carres.2009.11.026
AuthorSearch for: 1; Search for: 1
Affiliation
  1. National Research Council Canada. NRC Institute for Biological Sciences
FormatText, Article
Subjectlipopolysaccharide; Escherichia coli O:70; polysaccharide; antigen; Escherichia coli
Abstract
The structure of the antigenic O-polysaccharide (O-PS) produced by Escherichia coli serotype O:70 was determined by analysis of the chromatographically purified O-PS polymer prepared by mild hydrolysis of its aqueous phenol-extracted smooth-type somatic lipopolysaccharide. The O-PS is composed of d-glucose, d-galactose, d-fucose, 2-acetamido-2-deoxy-d-galactose, and 3-acetamido-3-deoxy-d-quinovose in a ratio of 1:1:1:1:1. From the use of DOC–PAGE, methylation, Smith-type periodate oxidation, and ¹H and ¹³C NMR spectroscopy, including 2D experiments, the O-PS was shown to be a polymer of a branched repeating pentasaccharide unit having the structure
Publication date
In
  • Carbohydrate Research 345, no. 5 (1 March 2010): 644–648.
LanguageEnglish
Peer reviewedYes
NPARC number15335895
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Record identifier10e49bf4-3eff-4a43-8284-d8c3cf32205f
Record created2010-05-21
Record modified2022-11-18

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