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Isolation and characterization of Methanobacterium espanolae sp. nov., a mesophilic, moderately acidiphilic methanogen

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1099/00207713-40-1-12
AuthorSearch for: 1; Search for: 1; Search for:
Affiliation
  1. National Research Council of Canada
FormatText, Article
Abstract
Bacterial strain GP9T (T = type strain), a nonmotile, nonsporeforming, mesophilic, methanogenic bacterium, was isolated from the primary sludge obtained from the waste treatment facility of a major kraft pulp mill in Canada. Single cells were 6.0 by 0.8 μm and stained gram positive. Growth and methane production occurred only with H2-CO2 as the substrate. Acetate, formate, propionate, butyrate, pyruvate, methanol, or trimethylamine could not serve as a sole source of carbon and energy for growth. The optimum pH for growth was between 5.6 and 6.2; consistent growth and methane production were not observed below pH 4.68. The optimum temperature for growth was 35°C, and little or no growth was observed during incubation at 15 and 50°C. Kanamycin and bacitracin were severe inhibitors of growth and methanogenesis, whereas 100 μM bromoethanesulfonic acid caused 30% inhibition. Supernatant from primary sludge enhanced growth by about 10%. The DNA base composition was 34 mol% guanine plus cytosine. On the basis of physiological characteristics, indirect immunofluorescence typing, and DNA-DNA hybridization studies, the isolate is named Methanobacterium espanolae sp. nov.
Publication date
In
  • International Journal of Systematic Bacteriology 40, no. 1 (1990): 12–18.
LanguageEnglish
NRC numberPATEL1990A
NRCC 30887
NPARC number9388904
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