| DOI | Resolve DOI: https://doi.org/10.1023/A:1009660315970 |
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| Author | Search for: Maqbool, S. B.; Search for: Husnain, T.; Search for: Riazuddin, S.; Search for: Masson, L.1; Search for: Christou, P. |
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| Name affiliation | - National Research Council of Canada. NRC Biotechnology Research Institute
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| Format | Text, Article |
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| Subject | Bacillus thuringiensis (Bt; cry2A; particle bombardment; rice leaf folder; transgenic rice; yellow stem borer |
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| Abstract | Transgenic rice indica varieties Basmati 370 and M 7 expressing the novel cry2A (Bt) insecticidal gene were generated by particle bombardment. Molecular and biochemical analyses in R0 and R1 populations confirmed stable integration and expression of this novel Bt transgene. We estimated that the gene product was expressed up to 5% of total leaf protein. Insect feeding bioassays demonstrated that the Cry2A protein was effective against the yellow stem borer and the rice leaf folder, two major rice pests in the Indian Subcontinent. This is the first report of the control of major rice pests using this specific Bt gene. The cry2A gene can now be used in combination with other insecticidal genes for pyramiding resistance against insect pests. This will delay, or perhaps in combination with integrated pest management practices, prevent evolution of insect populations resistant to single insecticidal genes. |
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| Publication date | 1998 |
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| In | |
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| Language | English |
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| NRC number | 41844 |
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| NPARC number | 3538689 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 0f3799e3-0d82-4fa7-b9f2-6fe4afb5147a |
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| Record created | 2009-03-01 |
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| Record modified | 2020-03-20 |
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