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The influence of antibody affinity on the radioallergosorbent test (RAST) and in vitro histamine release: Studies with hapten-specific monoclonal IgE antibodies

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1016/0022-1759(86)90365-0
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FormatText, Article
Subjectanibody affinity; monoclonal anitbody; IgE; RAST; histanmine release
Abstract
Four murine monoclonal IgE antibodies specific for the hapten, 4-hydroxy-3-nitrophenylacetyl (NP), had been previously found to be heteroclitic in nature in that they bound the crossreacting hapten, 4-hydroxy-3-iodo-5-nitrophenylacetyl (NIP), with greater affinity than NP. The influence of antibody affinity on the results of two commonly used assays for IgE, namely the radioallergosorbent test (RAST) and histamine release from rat peritoneal mast cells, was studied using these antibodies. In general, in agreement with previous reports, it was found that affinity influences both RAST and histamine release; however, the affinity constants deduced from equilibrium dialysis measurements for the reactions with monovalent haptens were not directly related to the activities of the antibodies as reflected in assays using multivalent hapten protein conjugates.
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In
  • Journal of Immunological Methods 89, no. 2 (22 May 1986): 249–255.
LanguageEnglish
Peer reviewedYes
NRC publication
This is a non-NRC publication

"Non-NRC publications" are publications authored by NRC employees prior to their employment by NRC.

NRC number1682
NPARC number9742064
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Record identifier0ec967c2-3fb9-46a5-9b70-784e135e636c
Record created2009-07-17
Record modified2020-03-17
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