| DOI | Resolve DOI: https://doi.org/10.1016/0304-4211(78)90070-6 |
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| Author | Search for: Huber, J.; Search for: Constabel, F.1; Search for: Gamborg, O. L1 |
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| Affiliation | - National Research Council of Canada. Prairie Regional Laboratory
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| Format | Text, Article |
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| Abstract | A method was developed to determine the cell number of cell aggregates and embryos. The method was employed to study the rate of cell proliferation and frequency of embryo formation in a cell suspension culture of anise (Pimpinella anisum L.). The efficiency of embryogenesis was affected by the initial cell concentration (population density). In addition, a close relationship existed between the rate of cell division in cell aggregates and the efficiency of embryo-genesis. Maximum embryogenic efficiency and rate of cell division occurred in cultures with an initial population density of 10⁵ cells/ml. Cell proliferation in creased several-fold during the transition from the “heart-stage” to the “torpedo-stage”. This burst of cell division was preceded by a transient rise of the average cell volume. |
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| Publication date | 1978-08 |
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| Publisher | Elsevier |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| Identifier | 0304421178900706 |
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| NRC number | NRCC-16682 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 0b976244-60e7-4e79-b88f-c9e2a3081d96 |
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| Record created | 2023-07-06 |
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| Record modified | 2023-07-11 |
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