| Alternative title | Metabolic interactions between the Lands Cycle and the Kennedy Pathway of Plant Glycerlipid synthesis |
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| DOI | Resolve DOI: https://doi.org/10.1105/tpc.112.104604 |
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| Author | Search for: Wang, Liping1; Search for: Shen, Wenyun1; Search for: Kazachkov, Michael1; Search for: Chen, Guanqun; Search for: Chen, Qilin2; Search for: Carlsson, Anders S.; Search for: Stymne, Sten; Search for: Weselake, Randall J.; Search for: Zou, Jitao1 |
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| Affiliation | - National Research Council of Canada. Aquatic and Crop Resource Development
- National Research Council of Canada. NRC Plant Biotechnology Institute
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| Format | Text, Article |
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| Abstract | It has been widely accepted that the primary function of the Lands cycle is to provide a route for acyl remodeling to modify fatty acid (FA) composition of phospholipids derived from the Kennedy pathway. Lysophosphatidylcholine acyltransferase (LPCAT) is an evolutionarily conserved key enzyme in the Lands cycle. In this study, we provide direct evidence that the Arabidopsis thaliana LPCATs, LPCAT1 and LPCAT2, participate in the Lands cycle in developing seeds. In spite of a substantially reduced initial rate of nascent FA incorporation into phosphatidylcholine (PC), the PC level in the double mutant lpcat1 lpcat2-2 remained unchanged. LPCAT deficiency triggered a compensatory response of de novo PC synthesis and a concomitant acceleration of PC turnover that were attributable at least in part to PC deacylation. Acyl-CoA profile analysis revealed complicated metabolic alterations rather than merely reduced acyl group shuffling from PC in the mutant. Shifts in FA stereospecific distribution in triacylglycerol of the mutant seed suggested a preferential retention of saturated acyl chains at the stereospecific numbering (sn)-1 position from PC and likely a channeling of lysophosphatidic acid, derived from PC, into the Kennedy pathway. Our study thus illustrates an intricate relationship between the Lands cycle and the Kennedy pathway. |
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| Publication date | 2012-11-13 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | NRCC 54658 |
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| NPARC number | 21268461 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 0b793346-d947-47c9-998d-ec5857192b44 |
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| Record created | 2013-07-24 |
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| Record modified | 2020-04-21 |
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