Laser-Induced Noninvasive Vascular Injury Models in Mice Generate Platelet- and Coagulation-dependent Thrombi

From National Research Council Canada

DOI	Resolve DOI: https://doi.org/10.1016/S0002-9440(10)64117-X
Author	Search for: Rosen, Elliot. D.; Search for: Raymond, Sylvain; Search for: Zollman, Amy; Search for: Noria, Francisco; Search for: Sandoval-Cooper, Mayra; Search for: Shulman, Alexis; Search for: Merz, James L.; Search for: Castellino, Francis J.
Format	Text, Article
Abstract	A minimally invasive laser-induced injury model is described to study thrombus development in mice in vivo. The protocol involves focusing the beam of an argon-ion laser through a compound microscope on the vasculature of a mouse ear that is sufficiently thin such that blood flow can be visualized by intravital microscopy. Two distinct injury models have been established. The first involves direct laser illumination with a short, high-intensity pulse. In this case, thrombus formation is inhibited by the GPIIb/IIIa antagonist, G4120. However, the anticoagulants, hirulog, PPACK, and NapC2 have minimal effect. This indicates that thrombus development induced by this model mainly involves platelet interactions. The second model involves low-intensity laser illumination of mice injected with Rose Bengal dye to induce photochemical injury in the region of laser illumination. Thrombi generated by this latter procedure have a slower development and are inhibited by both anticoagulant and anti-platelet compounds.
Publication date	2001-05-01
Publisher	Elsevier
In	American Journal of Pathology 158: 1613–1622.
NRC publication	This is a non-NRC publication "Non-NRC publications" are publications authored by NRC employees prior to their employment by NRC.
NPARC number	12743879
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Record identifier	05526be3-8b8c-44a9-991a-7b39e9c1b8e0
Record created	2009-10-27
Record modified	2020-03-27

Date modified:: 2025-12-17