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Sulphur requirements for growth of Acetivibrio cellulolyticus

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1139/m82-117
AuthorSearch for: 1; Search for: 1; Search for: 2
Affiliation
  1. National Research Council Canada
  2. National Research Council Canada. NRC Institute for Biological Sciences
FormatText, Article
Abstract
The sulphur requirement for growth of Acetivibrio cellulolyticus was determined using a mineral salts basal medium reduced with titanium(III) citrate and supplemented with 1% (w/v) cellobiose and 0.05% (w/v) yeast extract. Inorganic sulphide (Na2Sāˆ™9H2O) in the range of 0.7–1.0 mM was found to support maximum growth whereas 8.0 mM sulphide caused complete inhibition. Of the other sulphur-containing compounds tested (cystine, sodium thioglycollate, methionine, glutathione, homocystine, S-methyl-L-cysteine, inorganic sulphate) cysteine-HCl at 10–15 mM concentration was the only one which supported maximum growth that was at least 80% of that obtained in the optimum sulphide medium. A modified medium incorporating the optimum inorganic sulphide concentration supported maximum growth that was about twice that obtained in a conventional medium reduced with cysteine-Na2S. The possible reasons for the inhibition of growth in defined media containing Na2S as the sole reducing agent and the sulphur source are discussed.
Publication date
In
  • Canadian Journal of Microbiology 28 (1982): 772ā€“777.
LanguageEnglish
NRC numberPATEL1982A
NRCC 20199
NPARC number9372042
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Record identifier03daa648-e011-43b8-ad6b-13bc685a9b55
Record created2009-07-10
Record modified2020-03-13
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