Language selection

Government of Canada / Gouvernement du Canada

Search

Application of an immunoaffinity-based preconcentration method for mass spectrometric analysis of the O-chain polysaccharide of Aeromonas salmonicida from in vitro- and in vivo-grown cells

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1111/j.1574-6968.2009.01612.x
AuthorSearch for: 1; Search for: 1; Search for: 2; Search for: ; Search for: 1; Search for: 1
Affiliation
  1. National Research Council of Canada. NRC Institute for Biological Sciences
  2. National Research Council of Canada. NRC Institute for Marine Biosciences
FormatText, Article
SubjectAeromonas salmonicida; MS; lipopolysaccharide; identification
Abstract
In this study, application of magnetic beads (Dynabeads) coated with Aeromonas salmonicida lipopolysaccharide-specific polyclonal antisera to MS-based characterization of bacterial lipopolysaccharides has been evaluated. The results showed that the affinity-based preconcentration strategy resulted in at least a 100-fold increase in the detection of sensitivity, affording direct capillary electrophoresis (CE)-MS analysis of A. salmonicida lipopolysaccharide O-chain polysaccharide from in vitro-cultured cells. Subsequent CE-MS analysis of in vivo-grown cells of A. salmonicida confirmed significant changes in the structure of the lipopolysaccharide O-chain polysaccharide as a result of in vivo cultivation.
Publication date
In
  • FEMS Microbiology Letters 295, no. 2 (28 April 2009): 148–155.
LanguageEnglish
Peer reviewedYes
NPARC number15329306
Export citationExport as RIS
Report a correctionReport a correction (opens in a new tab)
Record identifier033a462f-168e-40bb-b774-66d906c4a63d
Record created2010-05-21
Record modified2020-04-16
Date modified: