| Download | - View final version: Enhancing microspore embryogenesis in diploid potato (Solanum tuberosum L. ssp. tuberosum) germplasm (PDF, 1.2 MiB)
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| DOI | Resolve DOI: https://doi.org/10.1007/s11240-024-02768-8 |
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| Author | Search for: Ferrie, Alison M.R.1ORCID identifier: https://orcid.org/0000-0002-1597-142X; Search for: Brost, Jennifer M.1 |
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| Affiliation | - National Research Council Canada. Aquatic and Crop Resource Development
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| Funder | Search for: National Research Council Canada |
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| Format | Text, Article |
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| Subject | diploid potato; doubled haploidy; microspore culture; potato; Solanum tuberosum |
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| Abstract | Doubled haploidy (DH) technology has been utilized in cultivated tetraploid potato (Solanum tuberosum L. ssp. tuberosum) to accelerate crop improvement; however very little work has been done with the diploid species. Experiments were undertaken to improve microspore embryogenic response in the diploid germplasm. Several factors influencing embryogenic responses were evaluated. An increase in calcium nitrate, a reduction in the plant growth regulators 6-benzyladenine (BA) and α-naphthaleneacetic acid (NAA), as well as an incubation temperature of 28 °C resulted in an increase in callus production and, in some cases, embryo-like structures. Validation of the modified protocol was conducted with both diploid and tetraploid potato germplasm with responses from both diploid and tetraploid. Monoploid and di-haploid plants were also regenerated from these microspore-derived calli. |
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| Publication date | 2024-04-29 |
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| Publisher | Springer Nature |
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| Licence | |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| Identifier | 2768 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 02886dd0-1436-4bdc-aaed-d49f090172df |
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| Record created | 2024-09-16 |
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| Record modified | 2025-11-03 |
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